Multiple molecular forms of certain lysosomal enzymes separated by isoelectric focusing on cellulose acetate membranes.
作者信息
Farrell D F, MacMartin M P, Clark A F
出版信息
Clin Chim Acta. 1978 Oct 2;89(1):145-55. doi: 10.1016/0009-8981(78)90370-4.
PMID:30549
Abstract
Analytical isoelectric focusing on cellulose acetate membranes offers greater resolution of the enzyme pattern than does standard electrophoresis and offers numerous advantages over other support media in isoelectric focusing. A series of lysosomal enzymes, deficiencies of which cause lethal storage diseases in infants and children, can be shown to exist as multiple molecular forms by analytical isoelectrical focusing on cellulose acetate membranes. This analytical technique should be a valuable tool in the investigation of enzyme polymorphism and genetically determined enzyme deficiency diseases.
摘要
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引用本文的文献
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