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[厌氧菌分离与研究的简易方法]

[Simple method of isolation and study of anaerobic bacteria].

作者信息

Ieniştea C

出版信息

Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1982 Jan-Mar;27(1):29-34.

PMID:7100765
Abstract

The description is given of a simple procedure for the surface culture of anaerobic bacteria in Petri dishes, using for anaerobiosis pieces of cardboard imbibed in pyrogalic acid and potassium carbonate solution respectively, pressing the seeded medium in the Petri dish, which becomes air-tight. The efficiency of the procedure was confirmed by seeding Clostridium strains, different collection species, and by isolation and identification of sulfite-reducing strains from meat and meat products.

摘要

介绍了一种在培养皿中对厌氧菌进行表面培养的简单方法,即分别使用浸有焦性没食子酸和碳酸钾溶液的硬纸板片来创造厌氧环境,将接种后的培养基压入培养皿使其密封。通过接种梭菌菌株、不同的收集物种,以及从肉类和肉类产品中分离和鉴定亚硫酸盐还原菌株,证实了该方法的有效性。

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