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曲霉和绿青霉在大麦中产生赭曲霉毒素A:真菌生长、时间、温度和接种量的影响。

Production of ochratoxin A in barley by Aspergillus ochraceus and Penicillium viridicatum: effect of fungal growth, time, temperature, and inoculum size.

作者信息

Häggblom P

出版信息

Appl Environ Microbiol. 1982 May;43(5):1205-7. doi: 10.1128/aem.43.5.1205-1207.1982.

Abstract

Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also followed during the same period and under the same conditions. The data show that ochratoxin A could be detected 4 to 6 days after inoculation at 25 degrees C, and the maximal accumulation of ochratoxin A was observed 28 days after inoculation. After 28 days at 25 degrees C, the quantities of ochratoxin A were between 7 and 46 micrograms/g of grain. At 10 degrees C only P. viridicatum produced ochratoxin A. The results indicated that production of ochratoxin A is not associated with rapid increase of glucosamine in the barley.

摘要

将湿润的大麦分别接种来自产赭曲霉毒素A的赭曲霉和变幻青霉菌株的1.4×10³和1.4×10⁵个孢子。为了估计大麦中的真菌组织,在10℃和25℃下跟踪葡糖胺的量28天。在同一时期和相同条件下也跟踪赭曲霉毒素A。数据表明,在25℃接种后4至6天可检测到赭曲霉毒素A,接种后28天观察到赭曲霉毒素A的最大积累。在25℃下28天后,赭曲霉毒素A的量在7至46微克/克谷物之间。在10℃时,只有变幻青霉产生赭曲霉毒素A。结果表明,赭曲霉毒素A的产生与大麦中葡糖胺的快速增加无关。

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