从美洲商陆（垂序商陆）种子中分离纯化另一种抗病毒蛋白及其部分特性研究

The pokeweed antiviral protein, previously identified in two forms (PAP and PAP II) in the leaves of Phytolacca americana (pokeweed) [Obrig. Irvin & Hardesty (1973) Arch. Biochem. Biophys. 155, 278-289; Irvin, Kelly & Robertus (1980) Arch. Biochem. Biophys. 200, 418-425] is a protein that prevents replication of several viruses and inactivates ribosomes, thus inhibiting protein synthesis. 2. PAP is present in several forms in the seeds of pokeweed. One of them, which we propose to call 'pokeweed antiviral protein from seeds' (PAP-S) was purified in high yield (180 mg per 100 g of seeds) by chromatography on CM-cellulose, has mol.wt. 30 000, and is similar to, but not identical with. PAP and PAP II. 3. PAP-S inhibits protein synthesis in a rabbit reticulocyte lysate with an ID50 (concentration giving 50% inhibition) of 1.1 ng/ml (3.6 x 10(-11) M), but has much less effect on protein synthesis by whole cells, with an ID50 of 1 mg/ml (3.3 x 10(-5) M), and inhibits replication of herpes simplex virus type 1.

商陆抗病毒蛋白先前在美洲商陆（商陆）叶片中被鉴定出两种形式（PAP和PAP II）[奥布里格、欧文和哈迪斯蒂（1973年）《生物化学与生物物理学文献》155卷，第278 - 289页；欧文、凯利和罗伯特斯（1980年）《生物化学与生物物理学文献》200卷，第418 - 425页]，它是一种能阻止多种病毒复制并使核糖体失活从而抑制蛋白质合成的蛋白质。2. 商陆种子中存在多种形式的PAP。其中一种，我们提议称为“种子来源的商陆抗病毒蛋白”（PAP - S），通过CM - 纤维素柱层析以高产率（每100克种子180毫克）纯化得到，分子量为30000，与PAP和PAP II相似但不相同。3. PAP - S在兔网织红细胞裂解物中抑制蛋白质合成，其ID50（产生50%抑制的浓度）为1.1纳克/毫升（3.6×10⁻¹¹摩尔/升），但对全细胞蛋白质合成的影响小得多，ID50为1毫克/毫升（3.3×10⁻⁵摩尔/升），并且能抑制单纯疱疹病毒1型的复制。

Purification and partial characterization of another form of the antiviral protein from the seeds of Phytolacca americana L. (pokeweed).