Green C J, Simpkin S, Grimaldi G, Johnson A
Br J Obstet Gynaecol. 1982 Aug;89(8):645-51. doi: 10.1111/j.1471-0528.1982.tb04720.x.
Initially, techniques for autografting ovaries with or without adnexa were developed but a 30% vascular failure rate was experienced by day 14. Of the technically successful grafts, 50% proved fertile. Single vascularized ovaries with their oviducts were then allografted into bilaterally ovariectomized rabbits by microsurgical techniques and the vascular failure rate was reduced to 5% of 40 grafts. The time-course of rejection in untreated recipients was mapped by histological examination and by 24-h culture of fallopian tubes after autopsy at different times after transplantation. Control allografts were consistently rejected by day 20. Striking prolongation of both types of graft was obtained with a short 17-day course of cyclosporin A at 10 or 15 mg day-1 kg-1. Indeed, significant evidence of rejection was found in only two ovaries out of 20 so far examined histologically. Mating behaviour, ovulation, ciliary function and transport of ova appeared normal in 80% of the recipients as long as 18 weeks after stopping cyclosporin A treatment. Only one of the ovarian allografted rabbits has so far been mated and this produced seven normal young 126 days after transplantation. However, none of the five animals mated after being allografted with en bloc adnexa have so far become pregnant.
最初，人们开发了自体移植带或不带附件卵巢的技术，但在第14天时出现了30%的血管失败率。在技术上成功的移植中，50%被证明具有生育能力。然后，通过显微外科技术将带有输卵管的单个血管化卵巢同种异体移植到双侧卵巢切除的兔子体内，血管失败率降至40个移植中的5%。通过组织学检查以及在移植后不同时间进行尸检后对输卵管进行24小时培养，绘制了未治疗受体的排斥反应时间进程。对照同种异体移植在第20天时始终被排斥。使用10或15毫克/天·千克−1的环孢素A进行17天的短期疗程，两种类型的移植均获得了显著延长。事实上，在迄今为止进行组织学检查的20个卵巢中，只有两个发现了明显的排斥证据。在停止环孢素A治疗长达18周后，80%的受体的交配行为、排卵、纤毛功能和卵子运输似乎正常。到目前为止，只有一只接受卵巢同种异体移植的兔子进行了交配，这只兔子在移植后126天产下了7只正常幼崽。然而，在接受整块附件同种异体移植后进行交配的5只动物中，到目前为止没有一只怀孕。
