Joubert F J, Taljaard N
Eur J Biochem. 1978 Oct;90(2):359-67. doi: 10.1111/j.1432-1033.1978.tb12612.x.
Three toxins (CM-2, CM-11 and CM-12) were purified from Naja haje haje (Egyptian cobra) venom. Whereas toxin CM-11 contains 65 amino acid residues and five intrachain disulphide bridges, toxin CM-2 and CM-12 comprise, respectively, 61 and 62 residues but both contain four disulphide bridges. The complete primary structures of the three toxins have been established. The sequence and the invarient amino acid residues of CM-2 resemble those of part of a long neurotoxin, a short neurotoxin and a cytotoxin. The sequence of CM-11 reveals that it is a homologue of the neurotoxins and to some extent also a cytotoxin. The immunochemical properties and the sequences of CM-12 suggest that it is related to the cytotoxin group. Further, the sequences of CM-11 and CM-12 from Naja haje haje venom show a high degree of homology with those of the corresponding toxins isolated from NaJA annulifera or NaJA melanoleuca venoms.
从埃及眼镜蛇（Naja haje haje）毒液中纯化出了三种毒素（CM - 2、CM - 11和CM - 12）。毒素CM - 11含有65个氨基酸残基和5个链内二硫键，而毒素CM - 2和CM - 12分别包含61个和62个残基，但二者均含有4个二硫键。已确定了这三种毒素完整的一级结构。CM - 2的序列和不变氨基酸残基类似于一种长神经毒素、一种短神经毒素和一种细胞毒素部分的序列。CM - 11的序列表明它是神经毒素的同源物，在某种程度上也是一种细胞毒素。CM - 12的免疫化学性质和序列表明它与细胞毒素组有关。此外，埃及眼镜蛇毒液中的CM - 11和CM - 12序列与从环纹眼镜蛇（NaJA annulifera）或黑曼巴蛇（NaJA melanoleuca）毒液中分离出的相应毒素序列具有高度同源性。
