微绒毛对采用荧光漂白恢复技术进行的侧向扩散测量的影响。
Effect of microvilli on lateral diffusion measurements made by the fluorescence photobleaching recovery technique.
作者信息
Wolf D E, Handyside A H, Edidin M
出版信息
Biophys J. 1982 Jun;38(3):295-7. doi: 10.1016/S0006-3495(82)84561-X.
To consider the effect of surface microvilli on measurements of lateral diffusion by fluorescence photobleaching recovery, we have measured the diffusion of the lipid probe 3,3'-dihexadecyl indocarbocyanine iodide on the villated main body and unvillated budding polar body of unfertilized mouse eggs. On the main body we found D = (6.41 +/- 0.62) x 10(-9) cm(2)/s with (77.0 +/- 2.1)% recovery, and on the budding polar body we found D = (7.05 +/- 0.75) x 10(-9) cm(2)/s with (84.7 +/- 1.3)% recovery. We thus find only slight differences in diffusion in the two regions.
为了研究表面微绒毛对通过荧光漂白恢复技术测量侧向扩散的影响，我们测量了脂质探针3,3'-二十六烷基吲哚羰花青碘化物在未受精小鼠卵的有绒毛主体和无绒毛出芽极体上的扩散。在主体上，我们发现扩散系数D = (6.41 ± 0.62) × 10⁻⁹ cm²/s，恢复率为(77.0 ± 2.1)%；在出芽极体上，我们发现D = (7.05 ± 0.75) × 10⁻⁹ cm²/s，恢复率为(84.7 ± 1.3)%。因此，我们发现这两个区域的扩散仅有细微差异。
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