Heynen M J, Verwilghen R L
Cell Tissue Res. 1982;224(2):397-408. doi: 10.1007/BF00216882.
Early, intermediate and late erythroblasts and reticulocytes were studied by electron microscopic morphometry. The volumes of mitochondria, Golgi zone and autophagosomes, as well as the surface areas of membranes of rough endoplasmic reticulum (RER) and of mitochondrial cristae and the numbers of ribosomes per unit volume cytoplasm were calculated. the results revealed 3 phases in erythroid maturation: (1) early and intermediate erythroblasts, (2) late erythroblasts, and (4) reticulocytes. The only significant difference between early and intermediate proliferating erythroblasts was a decrease in the surface area of the RER in the latter. After the last mitotic division in late erythroblasts significant reductions occurred in the RER, the Golgi apparatus, in the mitochondria and the number of ribosomes. The numbers of mitochondria and ribosomes were further reduced at the reticulocyte stage (clustered ribosomes more rapidly than single ones). Morphometric analysis showed no evidence of degradation of erythroid mitochondria whilst they are free in the cytoplasm, but there was some evidence of degradation after their uptake into autophagosomes in late erythroblasts and in reticulocytes.
通过电子显微镜形态计量学对早幼红细胞、中幼红细胞、晚幼红细胞和网织红细胞进行了研究。计算了线粒体、高尔基体区和自噬体的体积，以及粗面内质网（RER）膜、线粒体嵴膜的表面积和每单位体积细胞质中的核糖体数量。结果揭示了红细胞成熟的三个阶段：（1）早幼红细胞和中幼红细胞，（2）晚幼红细胞，（4）网织红细胞。早幼红细胞和中幼增殖红细胞之间唯一显著的差异是后者RER表面积的减少。晚幼红细胞最后一次有丝分裂后，RER、高尔基体、线粒体和核糖体数量显著减少。在网织红细胞阶段，线粒体和核糖体数量进一步减少（成簇核糖体比单个核糖体减少更快）。形态计量分析表明，红细胞线粒体在细胞质中游离时没有降解的迹象，但在晚幼红细胞和网织红细胞中被自噬体摄取后有一些降解的证据。
