Takahashi M, Nagase S, Taniguchi Y
Jikken Dobutsu. 1978 Jul;27(3):299-303. doi: 10.1538/expanim1978.27.3_299.
A rapid method is described for the determination of biochemical variants of mice by Titan III cellulose acetate electrophoresis. Variants examined were hemoglobin beta-chain, serum esterase-1, malic enzyme (supernatant form), and isocitrate dehydrogenase (supernatant form). The present procedure can be recommended as a simplified method to analyze biochemical variants for checking of marker genes in inbred strains of mice.
本文描述了一种通过Titan III醋酸纤维素电泳测定小鼠生化变异体的快速方法。所检测的变异体包括血红蛋白β链、血清酯酶-1、苹果酸酶(上清液形式)和异柠檬酸脱氢酶(上清液形式)。本方法可作为一种简化方法推荐,用于分析生化变异体,以检测近交系小鼠中的标记基因。