Shah P C, Freier S, Park B H, Lee P C, Lebenthal E
Gastroenterology. 1982 Oct;83(4):916-21.
Duodenal fluid was collected from normal volunteers before and after stimulation with pancreozymin-cholecystokinin and secretin. Protein content, proteolytic enzyme activities, and antibody activities against cow's milk proteins, alpha-casein, and beta-lactoglobulin B, were measured in the duodenal fluid. After pancreozymin-cholecystokinin stimulation, immunoglobulin A and immunoglobulin M antibody activities rose to peak levels in 5-10 min. The increase in protein content and proteolytic enzyme activities after pancreozymin-cholecystokinin stimulation paralleled the increase in antibody activity against the two cow's milk proteins. Secretin, in spite of its known dilutional effect on duodenal fluid enzyme concentration, also produced a rise in immunoglobulin M and immunoglobulin A antibody activities. Only a slight increase in immunoglobulin G antibody activity was noted after both pancreozymin-cholecystokinin and secretin. It is suggested that, pancreozymin-cholecystokinin and secretin, in addition to their well-established effects on the release of digestive enzymes, also stimulate release of specific antibodies against food proteins. Release of antibody coincident with food intake may act in preventing the inadvertent absorption of antigenic food proteins.
在正常志愿者接受胰酶泌素 - 缩胆囊素和促胰液素刺激前后收集十二指肠液。测定十二指肠液中的蛋白质含量、蛋白水解酶活性以及针对牛奶蛋白、α - 酪蛋白和β - 乳球蛋白B的抗体活性。胰酶泌素 - 缩胆囊素刺激后，免疫球蛋白A和免疫球蛋白M抗体活性在5 - 10分钟内升至峰值水平。胰酶泌素 - 缩胆囊素刺激后蛋白质含量和蛋白水解酶活性的增加与针对两种牛奶蛋白的抗体活性增加平行。促胰液素尽管对十二指肠液酶浓度有已知的稀释作用，但也会使免疫球蛋白M和免疫球蛋白A抗体活性升高。胰酶泌素 - 缩胆囊素和促胰液素刺激后，仅观察到免疫球蛋白G抗体活性略有增加。有人提出，胰酶泌素 - 缩胆囊素和促胰液素除了对消化酶释放有公认的作用外，还刺激针对食物蛋白的特异性抗体的释放。与食物摄入同时发生的抗体释放可能有助于防止抗原性食物蛋白的意外吸收。
