酵母的核糖体蛋白基因含有间隔序列。

Ribosomal protein genes of yeast contain intervening sequences.

作者信息

Bollen G H, Molenaar C M, Cohen L H, van Raamsdonk-Duin M M, Mager W H, Planta R J

出版信息

Gene. 1982 Apr;18(1):29-37. doi: 10.1016/0378-1119(82)90053-1.

Abstract

From a colony bank of HindIII-generated yeast DNA fragments we have isolated a number of recombinant DNAs carrying genes for ribosomal proteins (e.g., S10, S16A, S20, S24, S31, S33, L16, L25 and L34) of the yeast Saccharomyces carlsbergensis. By electron microscopic analysis of the R-loops formed between various DNA fragments and yeast mRNA, we could locate the ribosomal protein genes on the physical maps of the cloned DNA fragments. The R-loop structures observed indicate that a number of the ribosomal protein genes contain an intervening sequence.

摘要

从用HindIII酶切产生的酵母DNA片段的文库中，我们分离出了许多携带卡尔斯伯酵母核糖体蛋白基因（如S10、S16A、S20、S24、S31、S33、L16、L25和L34）的重组DNA。通过对各种DNA片段与酵母mRNA之间形成的R环进行电子显微镜分析，我们能够在克隆的DNA片段的物理图谱上定位核糖体蛋白基因。观察到的R环结构表明，许多核糖体蛋白基因含有间隔序列。