Matossian-Rogers A, Rogers P, Ledbetter J A, Herzenberg L A
Immunogenetics. 1982;15(6):591-9. doi: 10.1007/BF00347053.
Various murine tumor lines were screened by FACS analysis for the surface antigens ThB and Ly-6.2. Positive cell lines were used for immunoprecipitation studies. A monoclonal ThB-specific antibody immunoprecipitated a unique acidic protein of approximately 16 000 daltons from several positive tumors and from concanavalin A (Con-A) and LPS activated splenic lymphocytes. Monoclonal Ly-6.2-specific antibody was used to immunoprecipitate a 33 500 dalton protein that was shown to exist in four similarly sized forms with different basic charges. In the course of these studies, the apparent molecular weight of the surface antigen T 30, immunoprecipitated with a monoclonal T 30-specific antibody from the cell line EL4, was found to be approximately 25 000 daltons.
通过荧光激活细胞分选术（FACS）分析对多种小鼠肿瘤细胞系进行表面抗原ThB和Ly-6.2的筛选。阳性细胞系用于免疫沉淀研究。一种单克隆ThB特异性抗体从几种阳性肿瘤以及伴刀豆球蛋白A（Con-A）和脂多糖（LPS）激活的脾淋巴细胞中免疫沉淀出一种独特的约16000道尔顿的酸性蛋白。单克隆Ly-6.2特异性抗体用于免疫沉淀一种33500道尔顿的蛋白，该蛋白显示以四种大小相似但碱性电荷不同的形式存在。在这些研究过程中，用单克隆T 30特异性抗体从细胞系EL4中免疫沉淀出的表面抗原T 30的表观分子量约为25000道尔顿。
