Egger G, Porta S, Gollmann D
Inflammation. 1982 Jun;6(2):147-59. doi: 10.1007/BF00916239.
Stacks of six membrane filters (8-mu pore size) were placed over the mouths of plastic tubes. The tubes were filled with casein (test) or NaCl (control) solution and implanted subcutaneously in white rats; each animal received one tube with casein and one control tube. After 12, 18, 24, and 36 h, the tubes were removed and the filters stained and examined microscopically. Immigrant neutrophils were found either individually or in spherical aggregations within the filter meshwork. Crucial factors in the formation of aggregates included the frequency of individual cells, the chemotactic milieu (casein or NaCl), the duration of exposure, and the location within the filter stacks. The size of the aggregates depended on the duration of exposure. The phenomenon of "neutrophil aggregation" is thought to participate in the formation of the granulocyte wall around a focus of inflammation.
将六叠膜过滤器（孔径8微米）放置在塑料管管口上。管中装满酪蛋白（测试）或氯化钠（对照）溶液，然后皮下植入白鼠体内；每只动物接受一根装有酪蛋白的管和一根对照管。12、18、24和36小时后，取出管子，对过滤器进行染色并显微镜检查。在滤网中发现单个或呈球形聚集的迁移中性粒细胞。聚集形成的关键因素包括单个细胞的频率、趋化环境（酪蛋白或氯化钠）、暴露持续时间以及在滤器叠层中的位置。聚集体的大小取决于暴露持续时间。“中性粒细胞聚集”现象被认为参与炎症灶周围粒细胞壁的形成。
