Goldstein I M, Malmsten C L, Kindahl H, Kaplan H B, Rådmark O, Samuelsson B, Weissmann G
J Exp Med. 1978 Sep 1;148(3):787-92. doi: 10.1084/jem.148.3.787.
Human peripheral blood polymorphonuclear leukocytes were stimulated to generate thromboxane B2 in a time- and concentration-dependent fashion upon exposure to serum-treated zymosan particles. Conversion by stimulated PMN of [14C] arachidonic acid to [14C]thromboxane B2 was confirmed by thin-layer radiochromatography, radio-gas chromatography, and mass spectrometry. Generation of thromboxane B2 was independent of platelet contamination and could be inhibited by the cyclooxygenase inhibitor, indomethacin. Cells rendered incapable of ingesting particles by treatment with cytochalasin B generated comparable amounts of thromboxane B2. These results suggest that human peripheral blood polymorphonuclear leukocytes synthesize thromboxanes in response to surface stimulation independently of phagocytosis.
当暴露于血清处理的酵母聚糖颗粒时，人外周血多形核白细胞会以时间和浓度依赖性方式被刺激产生血栓素B2。通过薄层放射色谱法、放射气相色谱法和质谱法证实了受刺激的多形核白细胞将[14C]花生四烯酸转化为[14C]血栓素B2。血栓素B2的产生与血小板污染无关，并且可被环氧化酶抑制剂吲哚美辛抑制。用细胞松弛素B处理而无法摄取颗粒的细胞产生了相当数量的血栓素B2。这些结果表明，人外周血多形核白细胞在受到表面刺激时会独立于吞噬作用合成血栓素。
