Panagides J, Brockman J A, Barg W F
Inflammation. 1982 Jun;6(2):201-5. doi: 10.1007/BF00916244.
Lymphokine-activated (LK+) and control (LK-) macrophages were cultured for 66 h and then pulsed with [14C]glucosamine. Uptake of [14C]glucosamine was greater in LK+ than in LK- cultures. If, after 66 h, the medium was replaced with fresh medium and then pulsed with either [14C]glucose or [14C]glucosamine, the uptake of isotope was greatly reduced compared to cultures with no change of medium. However, uptake of both radiolabeled substances was still found to be greater in LK+ cultures than in LK- cultures. Although uptake of both substances was enhanced by lymphokines, the uptake kinetics of each isotope was different. Under similar conditions the uptake of [3H]leucine was not enhanced by lymphokine activation. These data are interpreted to mean that LK+ macrophages are metabolically stimulated and utilize more glucose and glucosamine. The difference in kinetics implies a different utilization by macrophages for each substance.
将淋巴因子激活的（LK +）巨噬细胞和对照（LK -）巨噬细胞培养66小时，然后用[14C]葡萄糖胺脉冲处理。LK +培养物中[14C]葡萄糖胺的摄取量高于LK -培养物。如果在66小时后，将培养基换成新鲜培养基，然后用[14C]葡萄糖或[14C]葡萄糖胺脉冲处理，与未更换培养基的培养物相比，同位素的摄取量会大大降低。然而，LK +培养物中两种放射性标记物质的摄取量仍高于LK -培养物。虽然两种物质的摄取都被淋巴因子增强，但每种同位素的摄取动力学不同。在相似条件下，淋巴因子激活不会增强[3H]亮氨酸的摄取。这些数据被解释为意味着LK +巨噬细胞在代谢上受到刺激，并且利用更多的葡萄糖和葡萄糖胺。动力学上的差异意味着巨噬细胞对每种物质的利用方式不同。
