Collins S J, Fialkow P J
Int J Cancer. 1982 Jun 15;29(6):673-6. doi: 10.1002/ijc.2910290612.
AKR mice heterozygous at the X-linked phosphoglycerate kinase (PGK) locus were used in experiments to determine the number of cells from which spontaneous thymic leukemias (thymomas) develop. Because only one of the two X-chromosomes is active in XX somatic cells, thymic leukemias that are clonal should display either type A or type B PGK, but not both, while those that are multicellular in origin may exhibit both enzymes. Spontaneous thymomas from 19 PGK heterozygous animals expressed exclusively (11 tumors) or predominantly (8 tumors) a single enzyme in contrast to non-malignant tissue from these animals which expressed both enzyme types in approximately equal ratios. When primary tumors expressing a single or predominant enzyme type were transplanted, the transplanted tumors invariably displayed the PGK phenotype that predominated in the initial tumor indicating that the minor PGK component was not contributed by malignant cells. These results indicate that spontaneous AKR leukemias are clonal.
在X连锁磷酸甘油酸激酶（PGK）基因座上杂合的AKR小鼠被用于实验，以确定自发胸腺白血病（胸腺瘤）起源的细胞数量。由于在XX体细胞中两条X染色体只有一条是活跃的，克隆性的胸腺白血病应该只显示A型或B型PGK，而不是两者都显示，而那些起源于多细胞的白血病可能会同时显示两种酶。来自19只PGK杂合动物的自发胸腺瘤只（11个肿瘤）或主要（8个肿瘤）表达一种单一的酶，与此形成对比的是，这些动物的非恶性组织以大致相等的比例表达两种酶类型。当表达单一或主要酶类型的原发性肿瘤被移植时，移植的肿瘤总是显示出在初始肿瘤中占主导的PGK表型，这表明次要的PGK成分不是由恶性细胞产生的。这些结果表明，自发的AKR白血病是克隆性的。
