Stein J, Lester J, Fosten A, Shownkeen R C, Hartree A S
J Endocrinol. 1982 Aug;94(2):203-10. doi: 10.1677/joe.0.0940203.
This report describes some of the properties of a clinical-grade preparation of human growth hormone (hGH) extracted from acetone-preserved autopsy human pituitary glands and used in Great Britain from 1967 to 1980. Gel filtration of this hGH on Sephadex G-100 yielded (on a weight basis) an average of 48% of a high molecular weight fraction, 10% of an intermediate fraction expected to contain dimeric forms of the hormone and 33% of a fraction considered to be the hGH monomer. The immunoassay potency of the monomer fraction was twice that of the clinical-grade preparation and the amino-acid composition of the monomer fraction agreed well with that obtained from published hGH sequence data. The results of polyacrylamide-gel electrophoresis (under reducing and dissociating conditions) and amino-acid analysis of the high molecular weight fraction suggest that it contains around 30% of aggregated hGH as well as other material not separated from hGH by the purification procedure.
本报告描述了一种从丙酮保存的尸检人垂体中提取的临床级人生长激素（hGH）制剂的一些特性，该制剂于1967年至1980年在英国使用。将这种hGH在葡聚糖G - 100上进行凝胶过滤，（以重量计）平均得到48%的高分子量级分、10%的预期含有激素二聚体形式的中间级分以及33%的被认为是hGH单体的级分。单体级分的免疫测定效价是临床级制剂的两倍，并且单体级分的氨基酸组成与从已发表的hGH序列数据获得的结果非常吻合。聚丙烯酰胺凝胶电泳（在还原和解离条件下）以及高分子量级分的氨基酸分析结果表明，它含有约30%的聚集hGH以及其他在纯化过程中未与hGH分离的物质。
