Muchmore A V, Decker J M, Blaese R M
J Immunol. 1979 Mar;122(3):1146-51.
The mechanism by which non-immune mononuclear cells recognize invading foreign material and are activated for cytotoxic attack was studied in a model system employing human mononuclear cells, fresh plasma, and 51Cr-labeled xenogeneic target erythrocytes. In these experiments, fresh antibody-depleted plasma or mononuclear leukocytes alone were poorly cytotoxic to xenogenic erythrocytes. However, these target cells were rapidly lysed when both fresh antibody-depleted plasma and mononuclear cells were present in the assay. The plasma factor could not be removed by extensive absorption with the target cells, was present in plasma from hypogammaglobulinemic patients, was heat labile, and was sensitive to incubation with zymosan and cobra venom factor. The "antigen" specificity of this reaction was directed by the serum factor inasmuch as target cells autologous to the effector cells could be killed in the presence of antibody-depleted xenogeneic plasma, but not autologous plasma. These data suggest that an important mechanism for the recognition of "foreigness" by non-immune mononuclear cells is via interaction with a plasma component, possibly a factor related to serum complement.
在一个使用人单核细胞、新鲜血浆和51Cr标记的异种靶红细胞的模型系统中，研究了非免疫单核细胞识别入侵异物并被激活进行细胞毒性攻击的机制。在这些实验中，单独的新鲜去除抗体的血浆或单核白细胞对异种红细胞的细胞毒性很差。然而，当检测中同时存在新鲜去除抗体的血浆和单核细胞时，这些靶细胞会迅速被裂解。血浆因子不能通过与靶细胞广泛吸附而去除，存在于低丙种球蛋白血症患者的血浆中，对热不稳定，并且对与酵母聚糖和眼镜蛇毒因子孵育敏感。该反应的“抗原”特异性由血清因子指导，因为在去除抗体的异种血浆存在下，与效应细胞自体的靶细胞可以被杀死，但自体血浆则不能。这些数据表明，非免疫单核细胞识别“异物”的一个重要机制是通过与血浆成分相互作用，可能是一种与血清补体相关的因子。
