Zürcher G, Da Prada M
J Neurochem. 1982 Jan;38(1):191-5. doi: 10.1111/j.1471-4159.1982.tb10871.x.
A simple, rapid and reliable radiometric assay for the determination of catechol-O-methyltransferase activity is described. The method is based on the conversion of catechol to [3H]guaiacol by catechol-O-methyltransferase in the presence of Mg2+, adenosine deaminase and S-adenosyl L-[methyl-3H]methionine. Incubation and direct extraction of [3H]guaiacol into organic scintillation fluid, as well as counting, are performed in the same standard scintillation vial. The assay is easy to perform and more sensitive than previous analogous procedures. The method has been applied to the assay of catechol-O-methyltransferase activity in discrete brain areas and also peripheral organs of rat and in human erythrocytes.
本文描述了一种用于测定儿茶酚-O-甲基转移酶活性的简单、快速且可靠的放射性测定法。该方法基于在Mg2+、腺苷脱氨酶和S-腺苷-L-[甲基-3H]甲硫氨酸存在的情况下，儿茶酚-O-甲基转移酶将儿茶酚转化为[3H]愈创木酚。[3H]愈创木酚的孵育、直接萃取到有机闪烁液中以及计数均在同一个标准闪烁瓶中进行。该测定法易于操作，且比以往类似方法更灵敏。该方法已应用于大鼠离散脑区以及外周器官和人红细胞中儿茶酚-O-甲基转移酶活性的测定。
