Durrett L R, Ziegler M G
J Neurosci Res. 1980;5(6):587-98. doi: 10.1002/jnr.490050613.
This assay measures picogram quantities of catechol drugs and endogenous catecholamines in body tissues and fluids. The catechols are converted to their 3H-O-methyl metabolites during incubation with 3H-S-adenosylmethionine then separated by solvent extraction and thin-layer chromatography. Most drugs containing the catechol structure can be radiolabeled and separated from norepinephrine and epinephrine by this technique to provide simultaneous measurement of endogenous and exogenously administered catechols. The disposition of isoproterenol in tissues and fluids of man and experimental animals is measured to illustrate the utility of this assay. The reactivity of several commonly administered catechol drugs with COMT is described and the possible implications discussed.
该测定法可测量人体组织和体液中皮克量的儿茶酚类药物和内源性儿茶酚胺。在与³H-S-腺苷甲硫氨酸孵育过程中，儿茶酚会转化为其³H-O-甲基代谢物，然后通过溶剂萃取和薄层色谱法进行分离。大多数含有儿茶酚结构的药物都可以进行放射性标记，并通过该技术与去甲肾上腺素和肾上腺素分离，从而同时测量内源性和外源性给予的儿茶酚。通过测量异丙肾上腺素在人和实验动物的组织和体液中的分布情况，来说明该测定法的实用性。描述了几种常用儿茶酚类药物与儿茶酚-O-甲基转移酶（COMT）的反应性，并讨论了其可能的影响。
