大鼠脑单个多巴胺能神经元系统中高香草酸的质量碎片色谱测定：反应气相色谱法的应用
Mass fragmentographic determination of homovanillic acid in individual dopaminergic neuron systems of rat brain: application of reaction gas chromatography.
作者信息
Matsumoto T, Uchimura H, Hirano M, Kim J S, Nakahara T, Tanaka K
出版信息
J Neurochem. 1982 Jan;38(1):285-8. doi: 10.1111/j.1471-4159.1982.tb10884.x.
Homovanillic acid (HVA) levels of 12 discrete rat brain areas were determined by a mass fragmentographic method using the reaction gas chromatographic technique. The use of reaction gas chromatography increased the sensitivity for determination of HVA. The sensitivity of this method allows measurement of HVA in small amounts of brain tissue. The HVA levels in polar, medial, and lateral fields of prefrontal cortex, anterior cingulate cortex, septum, amygdala, A12, A13, and A14 dopaminergic neurons were 0.417 +/- 0.018 ng/mg protein, 0.689 +/- 0.004, 0.753 +/- 0.024, 0.496 +/- 0.029, 1.311 +/- 0.046, 0.555 +/- 0.008, 1.949 +/- 0.077, 1.109 +/- 0.112, and 0.489 +/- 0.019, respectively. The HVA levels in these areas are first reported in the present paper.
采用反应气相色谱技术的质量碎片谱法测定了大鼠12个离散脑区的高香草酸（HVA）水平。反应气相色谱的使用提高了HVA测定的灵敏度。该方法的灵敏度允许对少量脑组织中的HVA进行测量。前额叶皮质的极区、内侧区和外侧区、前扣带回皮质、隔区、杏仁核、A12、A13和A14多巴胺能神经元中的HVA水平分别为0.417±0.018 ng/mg蛋白质、0.689±0.004、0.753±0.024、0.496±0.029、1.311±0.046、0.555±0.008、1.949±0.077、1.109±0.112和0.489±0.019。这些区域的HVA水平在本文中首次报道。
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