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通过气液色谱法和电子捕获检测法对神经组织中3,4-二羟基苯乙酸、高香草酸和高异香草酸进行测定。

The estimation of 3,4-dihydroxyphenylacetic acid, homovanillic acid and homo-isovanillic acid in nervous tissue by gas-liquid chromatography and electron capture detection.

作者信息

Pearson J D, Sharman D F

出版信息

Br J Pharmacol. 1975 Jan;53(1):143-8. doi: 10.1111/j.1476-5381.1975.tb07342.x.

DOI:10.1111/j.1476-5381.1975.tb07342.x
PMID:164966
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1666273/
Abstract

1 A gas chromatographic method using electron capture detection is described for the estimation of three acidic metabolites of dopamine, 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid, HVA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-hydroxy-4-methoxyphenylacetic acid (homo-isovanillic acid, iso-HVA). The method is based on the formation of the trifluoroacetyl-hexafluoroisopropyl derivatives of the three acids. 2 The method has been applied to the estimation of DOPAC, HVA and iso-HVA in tissues from the central and peripheral nervous systems.

摘要
  1. 描述了一种使用电子捕获检测的气相色谱法，用于测定多巴胺的三种酸性代谢物，即4-羟基-3-甲氧基苯乙酸（高香草酸，HVA）、3,4-二羟基苯乙酸（DOPAC）和3-羟基-4-甲氧基苯乙酸（高异香草酸，iso-HVA）。该方法基于这三种酸的三氟乙酰-六氟异丙酯衍生物的形成。2. 该方法已应用于中枢和外周神经系统组织中DOPAC、HVA和iso-HVA的测定。

相似文献

1
The estimation of 3,4-dihydroxyphenylacetic acid, homovanillic acid and homo-isovanillic acid in nervous tissue by gas-liquid chromatography and electron capture detection.通过气液色谱法和电子捕获检测法对神经组织中3,4-二羟基苯乙酸、高香草酸和高异香草酸进行测定。
Br J Pharmacol. 1975 Jan;53(1):143-8. doi: 10.1111/j.1476-5381.1975.tb07342.x.
2
A sensitive gas-liquid chromatographic assay for homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) applied to twenty areas of the human brain.一种用于检测高香草酸（HVA）和3,4-二羟基苯乙酸（DOPAC）的灵敏气-液色谱分析法，应用于人类大脑的二十个区域。
Brain Res. 1976 Dec 10;118(1):161-6. doi: 10.1016/0006-8993(76)90853-2.
3
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Correlation between high-performance liquid chromatography and automated fluorimetric methods for the determination of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid in nervous tissue and cerebrospinal fluid.高效液相色谱法与自动荧光法测定神经组织和脑脊液中多巴胺、3,4-二羟基苯乙酸、高香草酸和5-羟吲哚乙酸的相关性
J Chromatogr. 1982 Dec 10;233:69-77. doi: 10.1016/s0378-4347(00)81732-6.
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Simultaneous determination of dopamine, DOPAC and homovanillic acid. Direct injection of supernatants from brain tissue homogenates in a liquid chromatography--electrochemical detection system.多巴胺、3,4-二羟基苯乙酸和高香草酸的同时测定。将脑组织匀浆的上清液直接注入液相色谱 - 电化学检测系统。
J Chromatogr. 1980 Dec 12;221(2):237-47.
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Analysis of cat retina for dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine and homovanillic acid.对猫视网膜中的多巴胺、二羟基苯乙酸、3-甲氧基酪胺和高香草酸进行分析。
Vet Res Commun. 1989;13(3):173-81. doi: 10.1007/BF00142041.
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Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector.采用带电化学检测器的高压液相色谱法测定脑中的甲硫氨酸脑啡肽、去甲肾上腺素、多巴胺、3,4-二羟基苯乙酸（DOPAC）和3-甲氧基-4-羟基苯乙酸（HVA）。
Res Commun Chem Pathol Pharmacol. 1982 Mar;35(3):421-42.
8
Proceedings: A gas-liquid chromatographic method for the estimation of the acidic metabolites of dopamine in cerebrospinal fluid and brain tissues.论文：一种用于测定脑脊液和脑组织中多巴胺酸性代谢产物的气液色谱法。
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Assessment of cerebrospinal fluid levels of dopamine metabolites by gas chromatography.通过气相色谱法评估脑脊液中多巴胺代谢物的水平。
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Regional rat brain levels of 3,4-dihydroxyphenylacetic acid and homovanillic acid: concurrent fluorometric measurement and influence of drugs.大鼠脑局部区域中3,4-二羟基苯乙酸和高香草酸的含量：荧光法同步测定及药物的影响
Eur J Pharmacol. 1976 Aug;38(2):281-91. doi: 10.1016/0014-2999(76)90331-9.

引用本文的文献

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Br J Pharmacol. 1976 Oct;58(2):272P.
2
An apparatus for measuring the density of obese patients [proceedings].一种用于测量肥胖患者密度的仪器[会议论文集]
J Physiol. 1977 May;267(1):14P-15P.
3
The effect of chronic lithium administration on dopamine metabolism in rat striatum.长期给予锂对大鼠纹状体多巴胺代谢的影响。
Psychopharmacology (Berl). 1978 Mar 1;56(2):163-6. doi: 10.1007/BF00431843.

本文引用的文献

1
PRECISE ULTRAMICRO DETERMINATION OF STEROID HORMONES BY COMBINED TLC AND GLC ANALYSIS.采用薄层色谱法和气相色谱法联用对甾体激素进行精确超微量测定。
J Am Oil Chem Soc. 1965 Apr;42:283-90. doi: 10.1007/BF02540130.
2
A discussion of the modes of action of drugs which increase the concentration of 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) in the striatum of the mouse.关于能提高小鼠纹状体中4-羟基-3-甲氧基苯乙酸（高香草酸）浓度的药物作用模式的讨论。
Br J Pharmacol Chemother. 1967 Aug;30(3):620-6. doi: 10.1111/j.1476-5381.1967.tb02168.x.
3
Changes in the metabolism of 3,4-dihydroxyphenylethylamine (dopamine) in the striatum of the mouse induced by drugs.药物诱导小鼠纹状体中3,4-二羟基苯乙胺（多巴胺）代谢的变化。
Br J Pharmacol Chemother. 1966 Nov;28(2):153-63. doi: 10.1111/j.1476-5381.1966.tb01881.x.
4
The effect of tropolone on the formation of 3,4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in the brain of the mouse.托酚酮对小鼠脑中3,4-二羟基苯乙酸和4-羟基-3-甲氧基苯乙酸形成的影响。
Br J Pharmacol. 1969 May;36(1):107-15. doi: 10.1111/j.1476-5381.1969.tb08308.x.
5
3,4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in the mouse striatum: a reflection of intra- and extra-neuronal metabolism of dopamine?小鼠纹状体中的3,4-二羟基苯乙酸和4-羟基-3-甲氧基苯乙酸：多巴胺细胞内和细胞外代谢的反映？
Br J Pharmacol. 1971 Jul;42(3):343-51. doi: 10.1111/j.1476-5381.1971.tb07118.x.
6
On the mode of action of reserpine on dopamine metabolism in the rat striatum.关于利血平对大鼠纹状体多巴胺代谢的作用方式。
Eur J Pharmacol. 1971 Jan;13(2):155-67. doi: 10.1016/0014-2999(71)90146-4.
7
A new derivative for the gas-liquid chromatographic determination of homovanillic acid.一种用于气液色谱法测定高香草酸的新衍生物。
Anal Biochem. 1972 Jun;47(2):592-600. doi: 10.1016/0003-2697(72)90153-4.
8
Cerebrospinal fluid homovanillic acid and iso-homovanillic acid: a gas-liquid chromatographic method.
J Neurochem. 1974 Mar;22(3):333-5. doi: 10.1111/j.1471-4159.1974.tb07596.x.
9
Separation and determination of urinary homovanillic acid and iso-homovanillic acid by gas-liquid chromatography and electron capture detection.
J Lab Clin Med. 1973 Nov;82(5):829-35.
10
[4-O-Methylated catecholamines: 3-hydroxy, 4-methoxyphenylacetic acid (homo-isovanillic acid, iso-HVA) in biological media: urine, cerebrospinal fluid, and brain tissue].
Clin Chim Acta. 1972 Oct;41:5-17. doi: 10.1016/0009-8981(72)90490-1.