Osberghaus U, Schönert H, Deuticke B
J Membr Biol. 1982;68(1):29-35. doi: 10.1007/BF01872251.
A new way of measuring high diffusional membrane permeabilities of intact erythrocytes is presented using THO and 14C-glycol as test solutes. The technique combines the theoretical approach used by Redwood, Rall and Perl (J. Gen. Physiol. 64:706-729, 1974) and an experimental procedure introduced by Wang (J. Am. Chem. Soc. 73:510-513, 1951), which greatly simplifies the performance of the experiments. Permeability coefficients obtained by the new technique compare well to data derived by the approaches hitherto available. In view of its simplicity our method may be appropriate for the serial experiments necessary to characterize the transport mechanisms of water and other highly permeable lipophilic nonelectrolytes and for studies on other single cell systems.
本文介绍了一种使用THO和14C-二醇作为测试溶质来测量完整红细胞高扩散膜通透性的新方法。该技术结合了Redwood、Rall和Perl（《普通生理学杂志》64:706 - 729，1974）所采用的理论方法以及Wang（《美国化学会志》73:510 - 513，1951）引入的实验程序，这极大地简化了实验操作。通过新技术获得的渗透系数与迄今可用方法得出的数据相当。鉴于其简单性，我们的方法可能适用于表征水和其他高渗透性亲脂性非电解质转运机制所需的系列实验以及对其他单细胞系统的研究。
