Lawaczeck R
Biophys J. 1984 Mar;45(3):491-4. doi: 10.1016/S0006-3495(84)84184-3.
A light-scattering technique used to measure the water permeability across closed biomembranes is described, which is based on the different indices of refraction of D2O and H2O. This transient technique is compared with a similar method using D2O-sensitive fluorophores in the intravesicular space. The results of both techniques are equivalent although the signal-to-noise ratio favors the light-scattering or turbidity experiment. The light-scattering method is only applicable to larger particles (no point-scatterers) and is easily extended to biological objects. Data on the H2O/D2O exchange across membranes of ghosts from human erythrocytes suggest two mechanisms: the D2O and H2O permeation through the membrane and a slower D2O-induced conformational change of membraneous proteins.
描述了一种用于测量跨封闭生物膜水渗透性的光散射技术，该技术基于重水（D2O）和水（H2O）不同的折射率。将这种瞬态技术与在囊泡内空间使用对D2O敏感的荧光团的类似方法进行了比较。尽管信噪比有利于光散射或浊度实验，但两种技术的结果是等效的。光散射方法仅适用于较大颗粒（无点散射体），并且很容易扩展到生物对象。关于人红细胞空壳膜上H2O/D2O交换的数据表明有两种机制：D2O和H2O透过膜的渗透以及D2O诱导的膜蛋白构象变化较慢。
