Fleming H, Gurpide E
J Steroid Biochem. 1982 Jun;16(6):717-20. doi: 10.1016/0022-4731(82)90025-5.
The growth rate of stromal cells derived from 23 specimens of human endometrial tissue taken at various phases of the cycle was measured during the first seven days of primary culture. The stromal cells were found to differ radically in their ability to grow in culture according to the phase of the cycle at which the tissue was obtained. Early and mid proliferative stromal cells were capable of proliferation but that capacity was lost in late proliferative tissue. Growth at significant rates reappeared at days 21--22 and persisted in cells from late secretory tissue. Following plating, a 2--4 day lag period preceded proliferation of all but the most rapidly growing cultures. Subcultures showed a shorter lag period and faster maximal growth rate than the primary cultures from which they were derived. Estradiol, 10(-9) M. inhibition stromal cell growth in primary culture and this effect was prevented by 10(-8) M progesterone. These observations indicate that proliferative characteristics of stromal cells in primary culture are strongly influenced by the hormonal environment of the endometrium at the time of biopsy.
在原代培养的前七天，测量了取自月经周期不同阶段的23份人子宫内膜组织的基质细胞的生长速率。结果发现，根据获取组织时的月经周期阶段，基质细胞在培养中的生长能力有很大差异。增殖早期和中期的基质细胞能够增殖，但这种能力在增殖后期的组织中丧失。在第21 - 22天再次出现显著的生长速率，并在分泌晚期组织的细胞中持续存在。接种后，除生长最快的培养物外，所有培养物在增殖前都有2 - 4天的延迟期。传代培养物的延迟期比其来源的原代培养物短，最大生长速率更快。10(-9)M的雌二醇抑制原代培养中的基质细胞生长，而10(-8)M的孕酮可阻止这种作用。这些观察结果表明，活检时子宫内膜的激素环境对原代培养中基质细胞的增殖特性有强烈影响。
