Dorman B H, Varma V A, Siegfried J M, Melin S A, Adamec T A, Norton C R, Kaufman D G
In Vitro. 1982 Nov;18(11):919-28. doi: 10.1007/BF02796348.
Propagable cell cultures derived from human endometrial tissue were determined to contain cells predominantly of stromal cell origin based on their morphologic resemblance to endometrial stromal cells. These features included nexi, solitary cilia, and predecidual cytology. In addition to morphology the cell cultures retained a normal karyotype and responded to steroid hormones as evidenced by cellular aggregation. The stromal cells were evaluated for a variety of characteristics associated with transformed cells and seemed to be biologically normal without neoplastic phenotypes. Growth potential of the stromal cell cultures was also characterized in normal maintenance medium, in nutritionally depleted medium with reduced levels of calcium or serum, and in medium with increased levels of serum. The prolonged survival of the stromal cells in vitro coupled with the retention of in vivo characteristics and an absence of neoplastic phenotype provides a human cell system that is amenable to a variety of long-term experimental analyses.
从人子宫内膜组织衍生的可传代细胞培养物，根据其与子宫内膜基质细胞在形态上的相似性，被确定主要包含基质细胞来源的细胞。这些特征包括细胞连接、单根纤毛和蜕膜前细胞学特征。除形态学外，细胞培养物保持正常核型，并对类固醇激素有反应，细胞聚集证明了这一点。对基质细胞进行了与转化细胞相关的各种特征评估，它们似乎在生物学上是正常的，没有肿瘤表型。还在正常维持培养基、钙或血清水平降低的营养缺乏培养基以及血清水平升高的培养基中，对基质细胞培养物的生长潜力进行了表征。基质细胞在体外的长期存活，加上体内特征的保留和肿瘤表型的缺失，提供了一个适用于各种长期实验分析的人类细胞系统。
