温度变化对培养的人淋巴细胞中姐妹染色单体交换及细胞周期持续时间的影响

Effect of temperature variation on sister-chromatid exchange and cell-cycle duration in cultured human lymphocytes.

作者信息

Abdel-Fadil M R, Palmer C G, Heerema N

出版信息

Mutat Res. 1982 May-Jun;104(4-5):267-73. doi: 10.1016/0165-7992(82)90155-5.

Abstract

The relation of temperature variation and cell-cycle duration and their influence on SCE frequencies in human peripheral blood lymphocytes was studied. The results indicate that the frequency of SCEs is affected by variation in temperature. Either increase or decrease in the incubation temperature of cells leads to a higher frequency of SCEs than in control cultures grown at 37 degrees C. With a decrease in temperature to 35 degrees C the increase in SCE is accompanied by prolonged cell cycle. No significant difference in cell cycle was observed with the increase of temperature to 39 degrees C.

摘要

研究了温度变化与细胞周期持续时间的关系及其对人外周血淋巴细胞姐妹染色单体交换（SCE）频率的影响。结果表明，SCE频率受温度变化的影响。细胞培养温度的升高或降低都会导致SCE频率高于在37℃培养的对照培养物。当温度降至35℃时，SCE的增加伴随着细胞周期的延长。温度升高至39℃时，细胞周期未观察到显著差异。