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高度纯化的人B淋巴细胞和T淋巴细胞中的姐妹染色单体交换

Sister chromatid exchange in highly purified human B and T lymphocytes.

作者信息

Miller K

出版信息

Hum Genet. 1986 Feb;72(2):160-3. doi: 10.1007/BF00283937.

DOI:10.1007/BF00283937
PMID:3484717
Abstract

Sister chromatid exchange (SCE) rates were determined in human peripheral blood B and T lymphocyte populations highly purified by immunologic methods. The purified populations were supplemented with gamma-irradiated unseparated autologous mononuclear cells to restore helper-functions and stimulated with pokeweed mitogen (PWM) and phytohemagglutinin (PHA), respectively. Measured at the different peaks of proliferation after identical bromodeoxyuridine (BrdU) incubation times, T lymphocytes showed significantly higher SCE frequencies than B lymphocytes. In both populations, different proliferation kinetics and a different minimal BrdU concentration for sister chromatid differentiation (SCD) were observed.

摘要

采用免疫方法高度纯化人外周血B淋巴细胞群和T淋巴细胞群，测定姐妹染色单体交换（SCE）率。用γ射线照射未分离的自体单核细胞对纯化的细胞群进行补充，以恢复辅助功能，然后分别用商陆有丝分裂原（PWM）和植物血凝素（PHA）进行刺激。在相同的溴脱氧尿苷（BrdU）孵育时间后，在不同的增殖峰处进行测量，结果显示T淋巴细胞的SCE频率显著高于B淋巴细胞。在这两个细胞群中，均观察到不同的增殖动力学以及姐妹染色单体分化（SCD）所需的不同最低BrdU浓度。

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Sister chromatid exchange in highly purified human B and T lymphocytes.高度纯化的人B淋巴细胞和T淋巴细胞中的姐妹染色单体交换
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引用本文的文献

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SCE variability in lymphocytes and fibroblasts. A controlled study.淋巴细胞和成纤维细胞中的姐妹染色单体互换变异性。一项对照研究。
Hum Genet. 1988 Oct;80(2):117-23. doi: 10.1007/BF00702852.

本文引用的文献

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SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE.使用盐酸联苯胺的简化髓过氧化物酶染色法。
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Effect of temperature variation on sister-chromatid exchange and cell-cycle duration in cultured human lymphocytes.温度变化对培养的人淋巴细胞中姐妹染色单体交换及细胞周期持续时间的影响
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Relation between sister chromatid exchange, cell proliferation and proportion of B and T cells in human lymphocyte cultures.人淋巴细胞培养物中姐妹染色单体交换、细胞增殖与B细胞和T细胞比例之间的关系。
Hum Genet. 1981;57(1):31-4. doi: 10.1007/BF00271163.
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Trenimon-induced SCEs and structural chromosomal aberrations in early- and late-dividing lymphocytes.曲尼莫引起的早、晚期分裂淋巴细胞中的姐妹染色单体交换（SCEs）和染色体结构畸变。
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Cell kinetics and sister-chromatid-exchange frequency in human lymphocytes.
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Cell cycle-dependent regulation of excision repair of UV damage.紫外线损伤切除修复的细胞周期依赖性调控。
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Influence of various mitogens on the yield of sister-chromatid exchanges, induced by chemicals, in human lymphocytes.多种促细胞分裂剂对化学物质诱导的人淋巴细胞姐妹染色单体交换产率的影响。
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The stimulation of human B and T lymphocytes by various lectins.各种凝集素对人B淋巴细胞和T淋巴细胞的刺激作用。
Immunobiology. 1983 Aug;165(2):132-46. doi: 10.1016/S0171-2985(83)80055-2.
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Azathioprine, a clastogen in human somatic cells? Analysis of chromosome damage and SCE in lymphocytes after exposure in vivo and in vitro.
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Frequencies of sister-chromatid exchanges in relation to cell kinetics in lymphocyte cultures.淋巴细胞培养中姐妹染色单体交换频率与细胞动力学的关系
Mutat Res. 1980 May;70(3):343-50. doi: 10.1016/0027-5107(80)90024-x.