Kovács G L, Telegdy G, De Wied D
Neuropharmacology. 1982 May;21(5):451-4. doi: 10.1016/0028-3908(82)90030-2.
Various fragments of beta-lipotropin were micro-injected into the nucleus accumbens of free-moving rats and the effects on one-trial passive avoidance behaviour were studied. The following peptides were used: beta-endorphin (beta-LPH61-91), met-enkephalin (beta-LPH61-65), des-tyrosine-gamma-endorphin (DT gamma E, beta-LPH62-77), des-enkephalin-gamma-endorphin (DE gamma E, beta-LPH66-77), des-tyrosine-alpha-endorphin (DT alpha E, beta-LPH 62-76) and beta-LPH47-53(ACTH4-10). The gamma-type endorphins (DT gamma E and DE gamma E) were the only peptides which significantly attenuated passive avoidance behaviour when given in a 20 pg dose 1 hr before the 24-hr retention test. All the other peptides were ineffective. Microinjection of DT gamma E into the dentate gyrus of the dorsal hippocampus, on the other hand, was without effect on passive avoidance. The accumbens nucleus, therefore, is a preferential highly sensitive brain site for gamma-type endorphins. The attenuation of passive avoidance behaviour following systemic administration of these peptides might be the result of an interaction with this mesolimbic structure.
将β-促脂解素的各种片段微量注射到自由活动大鼠的伏隔核中，并研究其对单次被动回避行为的影响。使用了以下几种肽：β-内啡肽（β-LPH61-91）、甲硫氨酸脑啡肽（β-LPH61-65）、去酪氨酸-γ-内啡肽（DTγE，β-LPH62-77）、去脑啡肽-γ-内啡肽（DEγE，β-LPH66-77）、去酪氨酸-α-内啡肽（DTαE，β-LPH 62-76）和β-LPH47-53（促肾上腺皮质激素4-10）。在24小时记忆测试前1小时给予20皮克剂量时，γ型内啡肽（DTγE和DEγE）是唯一能显著减弱被动回避行为的肽。所有其他肽均无效。另一方面，将DTγE微量注射到背侧海马齿状回对被动回避没有影响。因此，伏隔核是γ型内啡肽优先作用的高敏感脑区。全身给予这些肽后被动回避行为的减弱可能是与这种中脑边缘结构相互作用的结果。
