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雏鸡发育过程中肠道半乳糖和亮氨酸转运的变化:低外部钙的影响

Changes in intestinal galactose and leucine transport during development in the chick: effect of low external calcium.

作者信息

Planas J M, Moreto M, Gaza E, Bolufer J

出版信息

Poult Sci. 1982 Jun;61(6):1094-8. doi: 10.3382/ps.0611094.

DOI:10.3382/ps.0611094
PMID:7111127
Abstract

There is some controversy in the literature as to the changes in sugar and amino acid absorption capacity by chicks during intestinal development in the posthatching period. For this reason, the transport of 5mM D-galactose and 4 mM L-leucine was studied in the mild intestine of the developing chick (range: 1 day to 35 days after hatch). In everted sacs, the net transepithelial transport of both substrates was maximal in the 1 to 3 days period and then it declined over the next 4 weeks. The final serosal:mucosal concentration ratio for galactose was 3.58 in 1 to 3-day-old chicks and fell to 1.62 in 26 to 30-day-old birds, while for leucine it fell from 3.52 to 1.50 in the same period. In intestinal rings from 1-day-old chicks, the uptake of 1 mM galactose after 1 min incubation was four times greater than in older animals. When the external calcium concentration was reduced from 2.5 mM (controls) to .1 mM the transport of galactose and leucine was markedly reduced: galactose transport was inhibited by 68 to 57% in 1 to 28-day-old chicks; leucine transport was inhibited by 82 to 50% in 1 to 7-day-old chicks with no significant effect thereafter.

摘要

关于雏鸡在孵化后肠道发育期间糖和氨基酸吸收能力的变化,文献中存在一些争议。因此,研究了发育中雏鸡(范围:孵化后1天至35天)小肠中5mM D-半乳糖和4mM L-亮氨酸的转运。在外翻肠囊实验中,两种底物的净跨上皮转运在1至3天期间最大,然后在接下来的4周内下降。1至3日龄雏鸡中半乳糖的最终浆膜:黏膜浓度比为3.58,在26至30日龄雏鸡中降至1.62,而亮氨酸在同一时期从3.52降至1.50。在1日龄雏鸡的肠段中,孵育1分钟后1mM半乳糖的摄取量比年长动物大4倍。当外部钙浓度从2.5mM(对照)降至0.1mM时,半乳糖和亮氨酸的转运明显减少:1至28日龄雏鸡中半乳糖转运受到68%至57%的抑制;1至7日龄雏鸡中亮氨酸转运受到82%至50%的抑制,此后无显著影响。

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Changes in intestinal galactose and leucine transport during development in the chick: effect of low external calcium.雏鸡发育过程中肠道半乳糖和亮氨酸转运的变化:低外部钙的影响
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Pflugers Arch. 1986 Aug;407(2):216-20. doi: 10.1007/BF00580679.