Planas J M, Villá M C, Ferrer R, Moretó M
Pflugers Arch. 1986 Aug;407(2):216-20. doi: 10.1007/BF00580679.
Hexose accumulation during development has been studied in tissue slices from chicken cecum. The age of birds ranged from 0 to 7 weeks after hatch. Ceca were divided into six portions according to their situation either proximal (PC), medial (MC) or distal (DC) to the ileocecal junction. In 0-day-old chicks all segments can accumulate 3-O-methyl-D-glucose (0.5 mmol/l) against a concentration gradient through a phloridzin-sensitive mechanism. Cumulative capacity is lower in DC than in PC and declines with development. Distal segments lose sugar transport ability 1-2 days after hatch whereas the medial region retains some concentrative ability in older birds. In 7-week chickens, PC slices have a similar cumulative ability to that of jejunum (yolk sac region). Kinetic studies showed that in PC the apparent Km for phloridzin-sensitive transport was half that in 1-day- than in 7-week-old birds; apparent Vm increased by 50% in this time range. The ability to transport sugars by the cecum was further confirmed in isolated enterocytes from 5- to 7-week-old chickens using alpha-methyl-D-glucoside (0.1 mmol/l) as substrate. Cell sugar concentration was greater in PC than in jejunal cells and jejunal greater than MC enterocytes. Sugar present in cells from DC was the same as in phloridzin-treated cells. It is concluded that cecal epithelium may play a significant role in the absorption of sugars during development.
已对鸡盲肠组织切片发育过程中的己糖积累情况进行了研究。鸡的年龄范围为孵化后0至7周。根据盲肠在回盲交界处近端（PC）、中间（MC）或远端（DC）的位置，将其分为六个部分。在0日龄雏鸡中，所有段都可以通过根皮苷敏感机制逆浓度梯度积累3-O-甲基-D-葡萄糖（0.5 mmol/L）。DC段的累积能力低于PC段，且随发育而下降。远端段在孵化后1-2天失去糖转运能力，而中间区域在大龄鸡中仍保留一些浓缩能力。在7周龄的鸡中，PC切片的累积能力与空肠（卵黄囊区域）相似。动力学研究表明，在PC段，根皮苷敏感转运的表观Km在1日龄鸡中是7周龄鸡的一半；在此时间范围内，表观Vm增加了50%。使用α-甲基-D-葡萄糖苷（0.1 mmol/L）作为底物，在5至7周龄鸡的分离肠细胞中进一步证实了盲肠转运糖的能力。PC段细胞中的糖浓度高于空肠细胞，空肠细胞中的糖浓度高于MC段肠细胞。DC段细胞中的糖与根皮苷处理细胞中的糖相同。得出的结论是，盲肠上皮在发育过程中可能在糖的吸收中起重要作用。
