Wu M, Wu D F, Wang X Q, Li S D, Luo X, Lu S X, Wang Y L, Ji C
Sci Sin B. 1982 Jul;25(7):738-44.
A new nitrosamine compound, N-1-methylacetonyl-N-3-methylbutyl-nitrosamine (MAMB-NA), isolated from corn bread inoculated with moulds commonly occurring in Linxian County, a high incidence area for esophageal cancer in North China was tested for its potential carcinogenicity in vitro. Baby hamster lung fibroblasts (BHL) were treated with MAMBNA in tissue culture medium containing liver microsome preparation of Aroclor 1254 treated rat. Cell transformation was assessed by morphological changes, loss of density-dependent inhibition and unlimited growth in vitro, chromosome changes, colony formation on soft agar, growth at low serum concentration, Con A agglutinability and tumor formation after heterotransplantation into immunosuppressed newborn rats. Like the known carcinogens N-methyl-N'-nitro-N-nitroso-guanidine and diethylnitrosamine, MAMBNA was able to cause malignant transformation of cells cultured in vitro.
从中国北方食管癌高发区林县常见霉菌接种的玉米面包中分离出一种新的亚硝胺化合物，即N-1-甲基丙酮基-N-3-甲基丁基亚硝胺（MAMB-NA），对其体外潜在致癌性进行了测试。在含有经Aroclor 1254处理的大鼠肝脏微粒体制剂的组织培养基中，用MAMBNA处理幼仓鼠肺成纤维细胞（BHL）。通过形态变化、密度依赖性抑制丧失和体外无限生长、染色体变化、软琼脂上的集落形成、低血清浓度下的生长、刀豆球蛋白A凝集性以及异种移植到免疫抑制新生大鼠后的肿瘤形成来评估细胞转化。与已知致癌物N-甲基-N'-硝基-N-亚硝基胍和二乙基亚硝胺一样，MAMBNA能够引起体外培养细胞的恶性转化。
