Court J P
Tropenmed Parasitol. 1982 Jun;33(2):83-6.
A new technique for examining the clinical prophylactic activity of antifilarial agents against Brugia pahangi is described. Third stage larvae were confined within diffusion chambers before intraperitoneal implantation into jirds. Such larvae developed normally within the chambers for 14 days when compared with infections in which larvae were allowed to grow freely within the peritoneal cavity. After this time conditions inside the chambers deteriorated and larval growth was inhibited. Larval recoveries from diffusion chambers after 14 days were higher and more reproducible than from intraperitoneal infections. The activities of known antifilarial agents against larvae confined within chambers implanted into jirds were examined. The benzimidazoles were found to be extremely effective Flubendazole killed almost all larvae when administered at 5 x 25 mg/kg sc by day 9 after the final treatment. It is suggested that the test described has potential as a screen for detecting clinical prophylactic activity because of its reproducibility, low parasite requirement, ease of parasite recovery and relatively short result acquisition time.
本文描述了一种检测抗丝虫药物对彭亨布鲁线虫临床预防活性的新技术。将第三期幼虫置于扩散小室中，然后腹腔植入沙鼠体内。与幼虫在腹腔内自由生长的感染相比，这些幼虫在小室内正常发育14天。14天后，小室内的条件恶化，幼虫生长受到抑制。14天后从扩散小室中回收的幼虫比腹腔感染回收的幼虫更多且更具可重复性。检测了已知抗丝虫药物对植入沙鼠体内小室中幼虫的活性。发现苯并咪唑类药物极其有效，在最后一次治疗后第9天，按5×25mg/kg皮下给药，氟苯达唑几乎杀死了所有幼虫。由于该试验具有可重复性、对寄生虫需求量低、寄生虫回收容易且结果获取时间相对较短，因此建议所描述的试验有潜力作为检测临床预防活性的筛选方法。
