Lupetti M, Dolfi A
Z Mikrosk Anat Forsch. 1982;96(2):214-20.
The esterase activity in the bursa of Fabricius in 10-day-old chicks is examined by means of serial sections obtained by cutting along the major axis of the lymphoid follicles. The positivity for this enzymatic reaction is not only confirmed to the FAE (follicle associated epithelial) cells of the epithelial frame-work and to isolated cells belonging to the medullary part of the follicle, but it extends in a club-shaped morphological continuity from the FAE cells towards the centre of the follicle. A similar image with a high affinity for acid stainings was also found by means of the H und E technique in suitably orientated sections. Morphological continuity between the FAE cells and the centre-medullary part leads us to suppose the FAE cells and the cells of the centre-medullary part may have a common origin. In the present work, we consider the possibility of FAE cells being of mesenchymatic origin because, besides the fact that they are in continuity with the centre-medullary cells, they appear after CFU (Colony Forming Units) migration, they have no basal lamina, they have certain features which are present in mesenchymatic cells belonging to the histiocytic line, like high esterase activity and micropinocytotic capability and, finally, they are sensitive to carrageenan and silica, which is a peculiarity of macrophages.
通过沿淋巴滤泡长轴切割获得的连续切片，对10日龄雏鸡法氏囊中的酯酶活性进行检测。这种酶促反应的阳性不仅在被证实存在于上皮框架的FAE（滤泡相关上皮）细胞以及属于滤泡髓质部分的单个细胞中，而且它以棒状形态连续从FAE细胞延伸至滤泡中心。通过苏木精-伊红（H&E）技术在适当取向的切片中也发现了对酸性染色具有高亲和力的类似图像。FAE细胞与中心髓质部分之间的形态连续性使我们推测FAE细胞和中心髓质部分的细胞可能有共同的起源。在本研究中，我们考虑FAE细胞起源于间充质的可能性，因为除了它们与中心髓质细胞连续之外，它们在集落形成单位（CFU）迁移后出现，它们没有基膜，它们具有属于组织细胞系的间充质细胞中存在的某些特征，如高酯酶活性和微胞饮能力，最后，它们对角叉菜胶和二氧化硅敏感，这是巨噬细胞的一个特性。
