Sher R, Anderson R, Glover A, Wadee A A
Infect Immun. 1978 Sep;21(3):959-65. doi: 10.1128/iai.21.3.959-965.1978.
Polymorphonuclear leukocyte motility, both in vivo and in vitro, and reduction of Nitro Blue Tetrazolium was studied in tuberculoid and lepromatous leprosy patients and a group of lepromatous patients with erythema nodosum leprosum (ENL). A profound defect in random migration, chemotaxis, and chemokinesis was found in lepromatous patients with and without complicating ENL, and marked depletion of skin window migration confirmed these in vitro findings. Tuberculoid patients exhibited a mild defect in polymorphonuclear leukocyte motility. Serum inhibitors of normal polymorphonuclear leukocyte chemotaxis were found in all types of leprosy, but sera from lepromatous and ENL patients were most inhibitory. Resting levels of Nitro Blue Tetrazolium reduction were normal in all three groups. Reconstitution of polymorphonuclear leukocyte cells from normal and ENL patients with ENL serum, however, showed increased Nitro Blue Tetrazolium reduction well above the normal range, whereas reconstitution with normal, lepromatous, and tuberculoid sera failed to increase Nitro Blue Tetrazolium reduction above the normal values.
对结核样型麻风、瘤型麻风患者以及一组伴有麻风结节性红斑（ENL）的瘤型麻风患者的多形核白细胞活力（体内和体外）及硝基蓝四氮唑还原情况进行了研究。在有或无并发ENL的瘤型麻风患者中发现随机迁移、趋化性和化学运动能力存在严重缺陷，皮肤窗迁移的显著减少证实了这些体外研究结果。结核样型患者的多形核白细胞活力存在轻度缺陷。在所有类型的麻风病中均发现了正常多形核白细胞趋化性的血清抑制剂，但瘤型和ENL患者的血清抑制作用最强。三组患者的硝基蓝四氮唑还原静息水平均正常。然而，用ENL血清重建正常和ENL患者的多形核白细胞后，硝基蓝四氮唑还原增加，远高于正常范围，而用正常、瘤型和结核样型血清重建则未能使硝基蓝四氮唑还原增加至正常水平以上。
