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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Kielty C M, Povey S, Hopkinson D A

Ann Hum Genet. 1982 May;46(2):135-43. doi: 10.1111/j.1469-1809.1982.tb00704.x.

The expression of soluble GPT (E.C. 2. 6. 1. 2) was analysed in seventeen independent rat hepatoma/human somatic cell hybrids and in forty-one subclones derived from two of these hybrids. 2. As judged by electrophoretic mobility, twelve hybrid clones expressed rat GPT activity only and three expressed strong rat and weak human GPT activity but no heteromeric isozymes. In the remaining two hybrids, only human GPT was demonstrable. 3. The segregation of GPT and marker enzymes in the primary hybrid cells and the subclones suggests that the human structural GPT locus is on chromosome 8. 4. The re-expression of rat GPT in segregating subclones derived from two primary hybrids which had extinguished this function, could not be correlated with presence or absence of any particular human chromosome(s).

在17个独立的大鼠肝癌/人类体细胞杂种以及从其中两个杂种衍生而来的41个亚克隆中分析了可溶性谷丙转氨酶（E.C. 2. 6. 1. 2）的表达情况。2. 根据电泳迁移率判断，12个杂种克隆仅表达大鼠谷丙转氨酶活性，3个克隆表达强大鼠和弱人类谷丙转氨酶活性，但无杂合同工酶。在其余两个杂种中，仅可检测到人类谷丙转氨酶。3. 谷丙转氨酶和标记酶在原代杂种细胞及亚克隆中的分离表明，人类结构性谷丙转氨酶基因座位于8号染色体上。4. 在两个已丧失该功能的原代杂种衍生的分离亚克隆中，大鼠谷丙转氨酶的重新表达与任何特定人类染色体的存在与否均无关联。

Kielty C M, Povey S, Hopkinson D A

Ann Hum Genet. 1982 May;46(2):135-43. doi: 10.1111/j.1469-1809.1982.tb00704.x.

The expression of soluble GPT (E.C. 2. 6. 1. 2) was analysed in seventeen independent rat hepatoma/human somatic cell hybrids and in forty-one subclones derived from two of these hybrids. 2. As judged by electrophoretic mobility, twelve hybrid clones expressed rat GPT activity only and three expressed strong rat and weak human GPT activity but no heteromeric isozymes. In the remaining two hybrids, only human GPT was demonstrable. 3. The segregation of GPT and marker enzymes in the primary hybrid cells and the subclones suggests that the human structural GPT locus is on chromosome 8. 4. The re-expression of rat GPT in segregating subclones derived from two primary hybrids which had extinguished this function, could not be correlated with presence or absence of any particular human chromosome(s).

在17个独立的大鼠肝癌/人类体细胞杂种以及从其中两个杂种衍生而来的41个亚克隆中分析了可溶性谷丙转氨酶（E.C. 2. 6. 1. 2）的表达情况。2. 根据电泳迁移率判断，12个杂种克隆仅表达大鼠谷丙转氨酶活性，3个克隆表达强大鼠和弱人类谷丙转氨酶活性，但无杂合同工酶。在其余两个杂种中，仅可检测到人类谷丙转氨酶。3. 谷丙转氨酶和标记酶在原代杂种细胞及亚克隆中的分离表明，人类结构性谷丙转氨酶基因座位于8号染色体上。4. 在两个已丧失该功能的原代杂种衍生的分离亚克隆中，大鼠谷丙转氨酶的重新表达与任何特定人类染色体的存在与否均无关联。