Eickbush T H, Kafatos F C
Cell. 1982 Jun;29(2):633-43. doi: 10.1016/0092-8674(82)90179-9.
We have recovered overlapping clones that represent in the aggregate a contiguous segment of chromosomal DNA 270 kb in length, or probably one third of the chorion locus of Bombyx mori. Approximately 70 genes have been identified, the majority of which are arranged in coordinately expressed pairs. The nonidentical genes expressed in the late period of choriogenesis are clustered within a single, 130 kb region, which is flanked by regions containing genes that are active during the middle developmental period. The late genes encode two families of high-cysteine proteins; the evolutionarily persistent clustering of these families contrasts sharply with the extensive sequence diversification of the structural genes and their flanking DNA elements. We discuss the possible regulatory significance of the clustered arrangement, as well as certain features of multigene family evolution.
我们已经获得了重叠克隆，这些克隆总体上代表了一段连续的染色体DNA片段，长度为270 kb，可能是家蚕绒毛膜基因座的三分之一。已鉴定出约70个基因，其中大多数基因以协同表达的对排列。在绒毛膜形成后期表达的不同基因聚集在一个130 kb的单一区域内，该区域两侧是含有在发育中期活跃的基因的区域。晚期基因编码两个高半胱氨酸蛋白家族；这些家族在进化上持续的聚类与结构基因及其侧翼DNA元件的广泛序列多样化形成鲜明对比。我们讨论了聚类排列可能的调控意义，以及多基因家族进化的某些特征。
