Madusa N, Kobayashi S, Mizuno K, Sakiyama F
J Biochem. 1978 Oct;84(4):971-5. doi: 10.1093/oxfordjournals.jbchem.a132211.
For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. By this method approximately 60% of the lysozyme in the urine was isolated in a pure state in ten days.
为了从白血病患者尿液中分离人溶菌酶，已建立了一种简单方法，该方法包括用60%饱和度的硫酸铵沉淀尿蛋白、在葡聚糖凝胶G-50上对粗溶菌酶进行分级分离以及通过CM-琼脂糖凝胶色谱法进行纯化。通过这种方法，在十天内约60%的尿液中的溶菌酶被纯态分离出来。
