Peeters T L, Depraetere Y R, Vantrappen G R
Clin Chem. 1978 Dec;24(12):2155-7.
We present a radioimmunoassay for lysozyme in human serum, based upon human lysozyme isolated from the urine of leukemic patients and antiserum prepared against this lysozyme in the goat. In the separation step, a second antibody is used. By properly adjusting the concentrations of unlabeled and 125I-labeled lysozyme and of the antibodies, maximal precision (SD, 0.04 mg/litre) was obtained in the range 0.00 to 2.00 mg/litre. In 20 normal volunteers the lysozyme concentration was 4.6 +/- 0.8 mg/litre (mean +/- SD), in 13 patients with monocytic leukemia 34.4 +/- 8.6 mg/litre. Correlation with lysoplate determinations was excellent in leukemic sera (r = 0.97) but was poor in normal sera (r = 0.35), possibly owing to the existence of isoenzymes.
我们提出了一种检测人血清中溶菌酶的放射免疫分析法，该方法基于从白血病患者尿液中分离出的人溶菌酶以及用此溶菌酶在山羊体内制备的抗血清。在分离步骤中，使用了第二抗体。通过适当调整未标记和125I标记的溶菌酶以及抗体的浓度，在0.00至2.00毫克/升的范围内获得了最高精度（标准差为0.04毫克/升）。20名正常志愿者的溶菌酶浓度为4.6±0.8毫克/升（平均值±标准差），13名单核细胞白血病患者的溶菌酶浓度为34.4±8.6毫克/升。白血病血清中与溶菌酶平板法测定的相关性极佳（r = 0.97），但正常血清中的相关性较差（r = 0.35），这可能是由于同工酶的存在。
