Nanikawa R, Ameno K, Hashimoto Y, Hamada K
Forensic Sci Int. 1982 Sep-Oct;20(2):133-40. doi: 10.1016/0379-0738(82)90138-4.
An experiment was carried out on rats to determine whether or not a skeletal muscle sample was suitable for the determination of ethanol concentration in a carcass. Gas chromatography was used to estimate the ethanol and n-propanol concentrations in the femoral muscle and intracardial blood. The ethanol concentration of each sample was corrected according to the moisture ratio of circulating blood, viz., 78.5%. The ethanol concentration ratio of blood to muscle was 1.03 two hours after ethanol administration. When the carcasses of rats pre-treated with ethanol were stored at 15 degrees C and 25 degrees C, respectively, the ethanol concentrations in muscle and blood increased with time. At all times the concentration was higher in blood than in muscle, and also higher in samples collected from the carcass stored at 25 degrees C than at 15 degrees C. When the control carcass was stored in the same manner, the postmortem production of ethanol was noticed in both blood and muscle. As in the experimental rats, the control rats exhibited a higher blood ethanol than muscle ethanol level. Again, the ethanol concentration was higher in samples collected from the carcass stored at 25 degrees C than at 15 degrees C. The ratio of ethanol to n-propanol was less than 20:1 in blood and less than 10:1 in muscle. These results suggest that skeletal muscle may be a suitable tissue for the postmortem detection of ethanol.
在大鼠身上进行了一项实验，以确定骨骼肌样本是否适合用于测定尸体中的乙醇浓度。使用气相色谱法估算股四头肌和心内血中的乙醇和正丙醇浓度。根据循环血液的含水率（即78.5%）对每个样本的乙醇浓度进行校正。乙醇给药两小时后，血液与肌肉中的乙醇浓度比为1.03。分别将预先用乙醇处理过的大鼠尸体储存在15℃和25℃时，肌肉和血液中的乙醇浓度随时间增加。在所有时间点，血液中的浓度均高于肌肉中的浓度，并且从储存在25℃的尸体中采集的样本中的浓度也高于储存在15℃的样本中的浓度。当以相同方式储存对照尸体时，在血液和肌肉中均发现了死后乙醇生成。与实验大鼠一样，对照大鼠的血液乙醇水平高于肌肉乙醇水平。同样，从储存在25℃的尸体中采集的样本中的乙醇浓度高于储存在15℃的样本中的乙醇浓度。血液中乙醇与正丙醇的比例小于20:1，肌肉中小于10:1。这些结果表明，骨骼肌可能是用于死后乙醇检测的合适组织。
