Imrei L, Sótonyi P
Int J Pediatr Otorhinolaryngol. 1982 Aug;4(3):215-24. doi: 10.1016/0165-5876(82)90003-9.
The upper respiratory tract of 20 infants and children, suffering from viral diseases, have been investigated cytochemically and electron microscopically. We employed the electron microscope negative staining and the concanavalin-A-peroxidase methods in our investigations, besides the usual serological and immunological ones. Concanavalin-A-peroxidase reaction showed a characteristic damaging of the cell-surface which may play an important role in viral infection. The electron microscope negative staining investigations showed adeno-, herpes- and influenza-virions which are referring to viruses. These morphological methods are useful and they increase the efficiency of diagnosing viral infections.
对20名患有病毒性疾病的婴幼儿的上呼吸道进行了细胞化学和电子显微镜检查。除了常规的血清学和免疫学方法外,我们在研究中还采用了电子显微镜负染色法和伴刀豆球蛋白A-过氧化物酶法。伴刀豆球蛋白A-过氧化物酶反应显示出细胞表面的特征性损伤,这可能在病毒感染中起重要作用。电子显微镜负染色检查显示了腺病毒、疱疹病毒和流感病毒颗粒,这些都与病毒有关。这些形态学方法很有用,它们提高了病毒感染的诊断效率。