Calvano S E, Mark D A, Good R A, Fernandes G
Immunopharmacology. 1982 Aug;4(4):291-302. doi: 10.1016/0162-3109(82)90050-9.
Spleen and lymph node cells from normal or adrenalectomized (Adx) rats were assessed for a variety of in vitro immunological measures, including mitogen responsiveness, antibody-dependent cellular cytotoxicity (ADCC), and natural killer (NK) cell activity. Proliferation in response to mitogens was evaluated with or without 2-mercaptoethanol (2-ME) in the culture media. Compared to spleen cells from normal rats, those from Adx rats demonstrated enhanced responsiveness to all mitogens tested. 2-ME increased proliferation of spleen cells from both normal and Adx rats, and the effect was additive to that of adrenalectomy. In contrast, lymph node cells from Adx rats manifested decreased mitogenic responsiveness compared to those of control rats and, with the addition of 2-ME, this difference disappeared for one of the mitogens tested (phytohemagglutinin). Compared to unoperated rats, ADCC and NK activities were significantly decreased in both the spleen and lymph nodes of the Adx rats. Alteration of normal leukocyte circulatory and homing patterns are suggested as a likely means by which adrenalectomy produces differential effects on lymphoid cells in various organs of the immune system.
对正常或肾上腺切除（Adx）大鼠的脾细胞和淋巴结细胞进行了多种体外免疫指标评估，包括丝裂原反应性、抗体依赖性细胞毒性（ADCC）和自然杀伤（NK）细胞活性。在培养基中添加或不添加2-巯基乙醇（2-ME）的情况下评估对丝裂原的增殖反应。与正常大鼠的脾细胞相比，Adx大鼠的脾细胞对所有测试丝裂原的反应性增强。2-ME增加了正常和Adx大鼠脾细胞的增殖，且该作用与肾上腺切除术的作用相加。相比之下，与对照大鼠相比，Adx大鼠的淋巴结细胞表现出丝裂原反应性降低，并且添加2-ME后，对于其中一种测试丝裂原（植物血凝素），这种差异消失。与未手术大鼠相比，Adx大鼠的脾脏和淋巴结中的ADCC和NK活性均显著降低。正常白细胞循环和归巢模式的改变被认为是肾上腺切除术对免疫系统各器官中的淋巴细胞产生不同影响的一种可能方式。
