Yang W C, Schultz R D
Dev Comp Immunol. 1986 Summer;10(3):405-18. doi: 10.1016/0145-305x(86)90030-3.
Cells from pigs of various ages were collected from peripheral blood, mesenteric lymph node, spleen and thymus and their ability to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) and natural killer (NK) cell activity was determined. ADCC against chicken red blood cell (CRBC) was present in cells from peripheral blood, lymph node and spleen, but was absent in thymic cells. There were no age-related differences in ADCC to CRBC and cells from fetal pigs had similar activities to cells from adult pigs. Maximal cytotoxicity against CRBC was found in the polymorphonuclear leukocyte (PMN) cell fraction. In contrast to the good response against CRBC, PMN cells were not lytic in the ADCC assay when PI3 virus-infected cells were used as target cells. Peripheral blood lymphocytes (PBL) had low but significant lytic activity against PI3 virus-infected cells in the presence of high concentrations of specific antiserum. NK cell activity against K562 target cells was readily detected in PBL of pigs older than 2 weeks but was not observed with cells from spleen, lymph node or thymus from pigs of any age. PBL of pigs younger than 2 weeks of age had low but detectable NK activity: however, fetal pigs had no NK activity against K562 target cells. In contrast, when PI3 virus infected Vero cells were used as target cells, NK cells were detected in spleen and PBL, but not lymph nodes or thymus, of pigs greater than one day of age. Similar to the absence of activity to K562, none of the lymphoid cells from fetal pigs had NK activity against PI3 virus-infected Vero cells. The present results suggest that the effector cells that mediate ADCC are distinct from those that mediate NK activity in that cells mediating ADCC develop earlier and are found in different organs than the NK cells. Additionally, the cells that mediate NK activity against viral infected cells may be different from those that mediate NK activity for K562 target cells; however, regardless of the target, NK cells are not present before birth in the pig.
采集不同年龄段猪的外周血、肠系膜淋巴结、脾脏和胸腺细胞，测定其介导抗体依赖性细胞介导的细胞毒性（ADCC）和自然杀伤（NK）细胞活性的能力。外周血、淋巴结和脾脏细胞存在针对鸡红细胞（CRBC）的ADCC，但胸腺细胞中不存在。针对CRBC的ADCC不存在年龄相关差异，胎儿猪的细胞与成年猪的细胞具有相似的活性。对CRBC的最大细胞毒性在多形核白细胞（PMN）细胞组分中发现。与对CRBC的良好反应相反，当使用PI3病毒感染的细胞作为靶细胞时，PMN细胞在ADCC试验中无细胞溶解作用。在高浓度特异性抗血清存在下，外周血淋巴细胞（PBL）对PI3病毒感染的细胞具有低但显著的细胞溶解活性。在2周龄以上猪的PBL中很容易检测到针对K562靶细胞的NK细胞活性，但在任何年龄猪的脾脏、淋巴结或胸腺细胞中均未观察到。2周龄以下猪的PBL具有低但可检测到的NK活性：然而，胎儿猪对K562靶细胞没有NK活性。相反，当使用PI3病毒感染的Vero细胞作为靶细胞时，在1日龄以上猪的脾脏和PBL中检测到NK细胞，但在淋巴结或胸腺中未检测到。与对K562无活性相似，胎儿猪的淋巴细胞均对PI3病毒感染的Vero细胞没有NK活性。目前的结果表明，介导ADCC的效应细胞与介导NK活性的细胞不同，因为介导ADCC的细胞比NK细胞发育更早且存在于不同器官中。此外，介导针对病毒感染细胞的NK活性的细胞可能与介导针对K562靶细胞的NK活性的细胞不同；然而，无论靶标如何，猪出生前均不存在NK细胞。
