Grofova I, Deniau J M, Kitai S T
J Comp Neurol. 1982 Jul 10;208(4):352-68. doi: 10.1002/cne.902080406.
The technique of intracellular recording and staining of the same neuron with horseradish peroxidase (HRP) was used to study the soma-dendritic and axonal morphology of nigrothalamic and nigrotectal cells in the rats. The nigrothalamic and nigrotectal cells were spread throughout the dorsoventral extent of the pars reticulata (SNR) and exhibited the same soma-dendritic and axonal features. Both populations consisted of medium-sized and large cells with extensive dendritic fields overlapping in all three directions. Their axons collateralized within the substantia nigra (SN) and in the mesencephalic tegmentum. The intrinsic collaterals were thin and branched partly within the dendritic field of a parent cell partly in remote regions of the SNR, and even in the pars compacta (SNC). The extrinsic branches involved thin arborizations in the rostroventral mesencephalic reticular substance and thicker descending and ascending collaterals. This material was supplemented by physiologically nonidentified HRP stained medium-sized and large neurons located in the SNR. The two kinds displayed the same extent and orientation of their dendrites but the branching patterns differed slightly. Proximal dendrites of all cells were coarse and smooth; thinner distal dendrites had varicosities and spinelike appendages. Some dendrites, specially those near the crus cerebri, terminated in dendritic thickets bearing many pleomorphic appendages. The orientation of dendritic fields varied with dorsoventral position of cells within the SNR. The most ventral region of the SNR contained neurons with dendrites oriented parallel to the crus cerebri and thus remained confined to the deepest stratum. The dendrites of cells in the central region of SNR were oriented mainly anteroposteriorly and ventrally, the ventral dendrites terminating in the ventralmost layer. Cells in the dorsolateral part of the SNR were characterized by the large dorsoventral extent of their dendrites which penetrated the entire thickness of SN. This variation in the arrangement of dendritic fields indicates that the SN is organized in three dorsoventral layers.
采用细胞内记录技术并用辣根过氧化物酶（HRP）对同一神经元进行染色，以研究大鼠黑质丘脑和黑质顶盖细胞的胞体 - 树突及轴突形态。黑质丘脑和黑质顶盖细胞分布于网状部（SNR）的背腹范围内，呈现出相同的胞体 - 树突及轴突特征。这两类细胞均由中等大小和大细胞组成，其广泛的树突野在三个方向上相互重叠。它们的轴突在黑质（SN）和中脑被盖内形成侧支。内在侧支较细，部分在母细胞的树突野内分支，部分在SNR的较远区域，甚至在致密部（SNC）分支。外在分支包括在腹侧中脑网状结构中的细分支以及较粗的下行和上行侧支。位于SNR的生理性质未明确的HRP染色的中等大小和大神经元补充了这些材料。这两类细胞的树突范围和方向相同，但分支模式略有不同。所有细胞的近端树突粗且光滑；较细的远端树突有曲张和棘状附属物。一些树突，特别是靠近大脑脚的那些，终止于带有许多多形附属物的树突丛。树突野的方向随SNR内细胞的背腹位置而变化。SNR最腹侧区域的神经元树突与大脑脚平行排列，因此局限于最深层。SNR中部区域细胞的树突主要向前、后和腹侧方向排列，腹侧树突终止于最腹侧层。SNR背外侧部分的细胞其树突在背腹方向上范围大，贯穿SN的整个厚度。树突野排列的这种变化表明SN在背腹方向上分为三层。
