Ryall R G, Chittleborough A J, Kane A
J Clin Pathol. 1978 Sep;31(9):878-82. doi: 10.1136/jcp.31.9.878.
Chloramphenicol (D-threo-2-dichloroacetamido-1-p-nitrophenylpropane-1,3-diol) added to blood samples did not alter the observed shift in the red cell osmotic fragility curves as the samples were aged in vitro for 24 hours at 37 degrees C. Nor was the normal rate of loss of 2,3-diphosphoglycerate, ATP, or glutathione from the red cells affected by the presence of chloramphenicol over the same period. Consequently, this bacteriostatic agent can be added to blood samples taken under non-sterile conditions, such as from the heel of an infant, in order to preserve them from the effects of microbial contamination. In this way red cell osmotic fragility results can be obtained on non-sterile samples after their incubation at 37 degrees C for 24 hours. A miniaturisation of the standard osmotic fragility procedure is described, which allows results to be produced from the small, non-sterile samples obtained by heel-prick of infants.
向血样中添加氯霉素（D-苏式-2-二氯乙酰胺基-1-对硝基苯基丙烷-1,3-二醇），当血样在37℃体外保存24小时时，并未改变所观察到的红细胞渗透脆性曲线的变化。在同一时期，红细胞中2,3-二磷酸甘油酸、三磷酸腺苷或谷胱甘肽的正常丢失率也不受氯霉素存在的影响。因此，这种抑菌剂可以添加到在非无菌条件下采集的血样中，比如从婴儿足跟采集的血样，以保护它们免受微生物污染的影响。通过这种方式，在非无菌样品于37℃孵育24小时后，就可以获得红细胞渗透脆性结果。本文描述了标准渗透脆性检测方法的小型化，该方法能够根据通过婴儿足跟采血获得的少量非无菌样品得出检测结果。
