人α（白细胞）干扰素在玻璃上的吸附：静电力和疏水力的作用

Adsorption of human alpha (leukocyte) interferon on glass: contributions of electrostatic and hydrophobic forces.

作者信息

Chadha K C, Sulkowski E

出版信息

J Interferon Res. 1982;2(2):229-34. doi: 10.1089/jir.1982.2.229.

DOI:10.1089/jir.1982.2.229

PMID:7119507

Abstract

Human leukocyte-derived interferon (HuIFN-alpha) can be displaced from controlled-pore glass (CPG) with alkylamines at 0.1-0.3 M concentration. The eluting power of an alkylamine correlates positively with the extent of its alkylation: CH3NH3 + less than (CH3)2NH2 + less than (CH3)3NH+ less than (CH3)4N+. Interferon can also be recovered using an electrolyte and ethylene glycol as cosolvents. Apparently, both hydrophobic and electrostatic forces are involved in the adsorption of HuIFN-alpha to CPG.

摘要

人白细胞衍生干扰素（HuIFN-α）在浓度为0.1 - 0.3 M时可被烷基胺从可控孔径玻璃（CPG）上置换下来。烷基胺的洗脱能力与其烷基化程度呈正相关：CH3NH3 + ＜ (CH3)2NH2 + ＜ (CH3)3NH+ ＜ (CH3)4N+。干扰素也可用电解质和乙二醇作为共溶剂进行回收。显然，HuIFN-α吸附到CPG上涉及疏水作用力和静电力。