Mecs I, Chin D, Fox F, Krim M
Arch Virol. 1984;81(3-4):303-11. doi: 10.1007/BF01310000.
Human leukocyte-derived alpha interferon [HuIFN-alpha(Le)] has been purified and/or concentrated on Carboxymethyl derivatized Controlled Pore Glass (CML-CPG240) beads. These glass beads adsorb HuIFN-alpha(Le) efficiently at acid pH and at physiological ionic strengths. Elution of HuIFN-alpha(Le) may be accomplished by several methods. Using buffers at relatively high ionic strengths (approximately 0.6 M) and pH values ranging from 2.6 to 6.9 for elution, preparations with specific activities of 10(5)-10(6) IU/mg were obtained with approximately 90 percent recoveries. Alternatively, using elution buffers at the same high ionic strength and at pH values ranging from 7.0 to 8.0, five-fold or better concentration and complete recovery of crude HuIFN-alpha(Le) were achieved. The resulting preparations were suitable for direct application to an antibody affinity chromatography column.
人白细胞衍生的α干扰素[HuIFN-α(Le)]已在羧甲基衍生的可控孔径玻璃（CML-CPG240）微珠上进行了纯化和/或浓缩。这些玻璃微珠在酸性pH值和生理离子强度下能有效吸附HuIFN-α(Le)。HuIFN-α(Le)的洗脱可以通过几种方法来完成。使用相对高离子强度（约0.6M）且pH值范围为2.6至6.9的缓冲液进行洗脱，可获得比活性为10(5)-10(6)IU/mg的制剂，回收率约为90%。或者，使用相同高离子强度且pH值范围为7.0至8.0的洗脱缓冲液，可实现粗制HuIFN-α(Le)五倍或更高倍数的浓缩以及完全回收。所得制剂适用于直接应用于抗体亲和色谱柱。
