Niwaguchi T, Kanda Y, Kishi T, Inoue T
J Forensic Sci. 1982 Jul;27(3):592-7.
A radioimmunoassay was developed for the determination of d-methamphetamine in urine. Antiserum to d-methamphetamine was prepared in rabbits by immunization with d-N-4-aminobutylmethamphetamine conjugated with bovine serum albumin. d-1-[3H]-Methamphetamine was used as a labeled compound for radioimmunoassay. The specificity of the antibody against d-methamphetamine was determined by cross-reaction studies with optical isomers of methamphetamine and its analogs. The antibody was specific for d-methamphetamine and exhibited no significant cross-reaction with the l-isomers. This stereoselective assay was applied to determination of d-methamphetamine excreted in urine after oral administration of d- or dl-methamphetamine to rats.
已开发出一种用于测定尿液中右旋甲基苯丙胺的放射免疫分析法。通过用与牛血清白蛋白偶联的右旋N-4-氨基丁基甲基苯丙胺免疫家兔，制备了针对右旋甲基苯丙胺的抗血清。右旋1-[3H]-甲基苯丙胺用作放射免疫分析的标记化合物。通过与甲基苯丙胺的光学异构体及其类似物的交叉反应研究，确定了抗右旋甲基苯丙胺抗体的特异性。该抗体对右旋甲基苯丙胺具有特异性，与左旋异构体无明显交叉反应。这种立体选择性分析方法用于测定大鼠口服右旋或消旋甲基苯丙胺后尿液中排泄的右旋甲基苯丙胺。
