Nagai T, Kamiyama S, Nagai T
Dept. of Legal Medicine, Dokkyo University, School of Medicine, Tochigi, Japan.
Z Rechtsmed. 1988;101(3):151-9. doi: 10.1007/BF00201110.
Optical isomers (d and l) and racemic compounds (dl) of methamphetamine (MAMP) and amphetamine (AMP), and biologic materials including those substances, could be analyzed by high performance liquid chromatography. Examining the temperature for the analysis, 40 degrees C was the optimal condition in the reproducibility of separated MAMP-isomers. The reproducibility at the temperature did not vary significantly. The measured values of optical isomers were 0.116 +/- 0.012, 1.082 +/- 0.070 and 8.984 +/- 0.136 for the mixing ratios (l/d) of 0.111, 1.000, and 9.000, respectively. The detection limit for both d- and l-isomers was 25 ng. The analytic result of hair specimens from two stimulant abusers by the present method indicates that they contained only d-MAMP and d-AMP, which is believed to have the strongest pharmacologic effect among the optical isomers of MAMP. The coefficient of variation in the analysis of five replicate standards, prepared by adding 1,000 ng each of racemate MAMP and AMP to hair, was less than 4%. The measured value against l/d = 1.000 was 1.040 +/- 0.040 in MAMP and 0.980 +/- 0.030 in AMP. The detection limit for both racemate MAMP and AMP accumulated in hair was 250 ng. The analysis of the optical isomers by our method would contribute to identifying the smuggling routes or the illicit method.
甲基苯丙胺（MAMP）和苯丙胺（AMP）的光学异构体（d型和l型）以及外消旋化合物（dl型），包括这些物质的生物材料，都可以通过高效液相色谱法进行分析。考察分析温度时，40℃是分离MAMP异构体重现性的最佳条件。该温度下的重现性没有显著变化。对于混合比（l/d）分别为0.111、1.000和9.000的情况，光学异构体的测量值分别为0.116±0.012、1.082±0.070和8.984±0.136。d型和l型异构体的检测限均为25 ng。用本方法对两名兴奋剂滥用者毛发样本的分析结果表明，他们的毛发中仅含有d-MAMP和d-AMP，据信这是MAMP光学异构体中药理作用最强的。向毛发中分别添加1000 ng外消旋MAMP和AMP制备的五个重复标准品分析的变异系数小于4%。对于l/d = 1.000，MAMP的测量值为1.040±0.040，AMP的测量值为0.980±0.030。毛发中积累的外消旋MAMP和AMP的检测限均为250 ng。用我们的方法分析光学异构体将有助于识别走私路线或非法方法。
