Kuehn K, Sunderman F W
J Inorg Biochem. 1982 Aug;17(1):29-39. doi: 10.1016/s0162-0134(00)80227-5.
Seventeen nickel compounds were incubated in water, rat serum, and rat renal cytosol for 72 hr at 37 degrees C. Concentrations of dissolved nickel were analyzed by electrothermal atomic absorption spectrophotometry, and dissolution half-times (T50) were computed by the Weibull equation. Eleven of the nickel compounds (Ni, beta NiS, amorphous NiS, alpha Ni3S2, NiSe, Ni3Se2, NiTe, NiAs, Ni11As8, Ni5As2, and NiFeS4) dissolved more rapidly in serum or cytosol than in water. Four of the compounds (NiO, NiSb, NiFe alloy, and NiTiO3) had no detectable dissolution in any of the media (i.e., T50 greater than 11 yr). One compound (NiAsS) had approximately equal T50 values in the three media; the T50 value of one compound (NiS2) could not be determined in serum or cytosol owing to precipitation. T50 value of 34 and 21 days for dissolution of alpha Ni3S2 in serum and cytosol, respectively, agree closely with the excretion half-time of 24 days derived from previously reported data for excretion of 63Ni in urine and feces of rats after intramuscular injection of alpha 63Ni3S2. These findings suggest that in vitro dissolution half-times of nickel compounds may be used to predict their in vivo excretion half-times, since the dissolution process is rate-limiting to their metabolism and excretion.
将17种镍化合物于37℃在水、大鼠血清和大鼠肾细胞溶质中孵育72小时。通过电热原子吸收分光光度法分析溶解镍的浓度，并通过威布尔方程计算溶解半衰期（T50）。11种镍化合物（Ni、β-NiS、非晶态NiS、α-Ni3S2、NiSe、Ni3Se2、NiTe、NiAs、Ni11As8、Ni5As2和NiFeS4）在血清或细胞溶质中的溶解速度比在水中更快。4种化合物（NiO、NiSb、NiFe合金和NiTiO3）在任何一种介质中均未检测到溶解（即T50大于11年）。一种化合物（NiAsS）在三种介质中的T50值大致相等；一种化合物（NiS2）由于沉淀而无法在血清或细胞溶质中测定T50值。α-Ni3S2在血清和细胞溶质中的溶解T50值分别为34天和21天，这与先前报道的肌肉注射α-63Ni3S2后大鼠尿液和粪便中63Ni排泄的24天排泄半衰期密切相符。这些发现表明，镍化合物的体外溶解半衰期可用于预测其体内排泄半衰期，因为溶解过程是其代谢和排泄的限速步骤。
